Cytosolic DNA Sensor Upregulation Accompanies DNA Electrotransfer in B16.F10 Melanoma Cells
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چکیده
منابع مشابه
Cytosolic DNA Sensor Upregulation Accompanies DNA Electrotransfer in B16.F10 Melanoma Cells
In several preclinical tumor models, antitumor effects occur after intratumoral electroporation, also known as electrotransfer, of plasmid DNA devoid of a therapeutic gene. In mouse melanomas, these effects are preceded by significant elevation of several proinflammatory cytokines. These observations implicate the binding and activation of intracellular DNA-specific pattern recognition receptor...
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BACKGROUND Tumor irradiation combined with adjuvant treatments, either vascular targeted or immunomodulatory, is under intense investigation. Gene electrotransfer of therapeutic genes is one of these approaches. The aim of this study was to determine, whether gene electrotransfer of plasmid encoding shRNA for silencing endoglin, with vascular targeted effectiveness, can radiosensitize melanoma ...
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The development of DNA vaccines requires appropriate delivery technologies. Electrotransfer is one of the most efficient methods of non-viral gene transfer. In the present study, intradermal DNA electrotransfer was first optimised. Strong effects of the injection method and the dose of DNA on luciferase expression were demonstrated. Pre-treatments were evaluated to enhance DNA diffusion in the ...
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Wynn H. Kao1,7, Adam I. Riker2, Deepa S. Kushwaha3, Kimberly Ng3, Steven A. Enkemann4, Richard Jove5, Ralf Buettner5, Pascal O. Zinn3, Néstor P. Sánchez1, Jaime L. Villa6, Alan D. D'Andrea3, Jorge L. Sánchez1, Richard D. Kennedy3, Clark C. Chen†,3, and Jaime L. Matta†,6 1Department of Dermatology, University of Puerto Rico School of Medicine, San Juan, PR 2Ochsner Cancer Institute, Ochsner Medi...
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ژورنال
عنوان ژورنال: Molecular Therapy - Nucleic Acids
سال: 2016
ISSN: 2162-2531
DOI: 10.1038/mtna.2016.34